Medical microbiology (classes) (ćwiczenia) - 2019/2020

Course description
General information
Lecturer:dr Monika Jach
Organising unit:Faculty of Science and Health - Instytut Nauk Biologicznych
Number of hours (week/semester): 2/30
Language of instruction:English
Course objective
C1. Upon completion of this course students will be able to describe: the biological differences between bacteria and yeasts, including many etiological agents responsible for infectious diseases
C2. It covers all biology of bacteria and other pathogens related with infectious diseases. Students will be able to explain the tests used by microbiologists to identify and characterize organisms.
C3. In order to foster a student’s ability to analyze and solve problems in their future industrial career, the course will provide the conceptual basis for understanding pathogenic microorganisms and particularly address the fundamental mechanisms of their pathogenicity.
C4. It will provide opportunities for a student to develop both informatics and diagnostic skills in microbiology, including the practical application and interpretation of laboratory tests for the identification and differentiation of microorganisms.
W1. This course requires that you have taken the following courses (or equivalent) in general microbiology and biochemistry.
W2. Perform a simple task research under the guidance of a tutor.
W3. Able to interact and work in a group.
Learning outcomes
W1. Define principles of microbial taxonomy, structure, physiology, immunology and pathogenesis. Develop knowledge of microbial organisms and their relevance of infectious diseases in human (K_W01, K_W02).
W2. Understand the principles of prevention of pathogenic microorganism’s infection and has a basic knowledge of the most important groups of microorganisms that cause infections in humans and their potential pathogen and its practical impact on various industries (K_W02).
W3. Define principles of planning and development of microbiological tests using the techniques and tools used in microbiology (K_W05, K_W06).
W4. Understand the basic principles of safety, hygiene and ergonomics, indicating the possibility of psychophysical in the work environment (K_W09).

U1 Familiar skill of the laboratory use in diagnose of microorganism, including appropriate specimen collection and selection of tests. Develop the ability to manipulate the laboratory tests to identify pathogenic microorganisms (K_U01, K_U02, and K_U05).
U2 able to use the light microscope, isolated prepare microscopic preparations, conduct and document the microscopic observation (K_U03).
U3 Understand and use the principles of the laboratory tests in diagnosis and identification of pathogenic microorganisms Collect and interpret the results of diagnostic tests, and on this basis, formulate appropriate proposals, able to prepare report (K_U01, K_U5).
U4 learn alone in a targeted manner within a range of issues of medical microbiology (K_U07).
U5 Understands and use the scientific literature in the field of medical microbiology in the English language, define the most important group of microorganisms that cause infections in humans, K_U08, K_U09,
U6 Prepare written issues related to medical microbiology in the English language using scientific language (K_U10).
K1. Understand the need to continuously deepen and update their knowledge and skills related to the field of microbial is open to the use of new microbiological techniques (K_K01).
K2 Take care for entrusted equipment, respect of self and others, shows willingness to collaborative problem solving and substantive discussion (K_K02).
K3 Act in accordance with the principles of health and safety and ethics in the practice of effectively using microbiological methods of destroy microorganisms, shows the responsibility for assessing the risks of applied research techniques, know how to act in emergency (K_K03).
K4 Understand the dilemmas associated with the development of microbiological pathogens and the social and economic importance of pathogenic microorganisms. It is aware of ethical conduct when planning and performing research experience K_K05.
Teaching method
Information lectures, discussion and practice exercises in the laboratory.
Course content description
This course will introduce students to the microbial species that cause human disease. This course will focus on mechanisms of microbial pathogenesis and the host response, and the scientific approaches that are used to investigate these processes. It will know organisation of work and occupational health and safety regulations applicable in microbiological laboratory. Review current systematics of the most important pathogenic microorganisms; including obligatory pathogens and opportunistic. It will know basic of differentiation and cultivation of microorganisms. It will prepare diagnosis specific pathogens using traditional methods. Isolation, identification and differentiation of microorganisms, including serological tests. Microbiological safety of medicines, foodstuffs, water and air, including the test methods of microbiological purity

Topics of Medical Microbiology exercises
Exercise 1. Theoretical and practice explanation of organisation of work and occupational health and safety regulations applicable in microbiological laboratory.
Exercise 2. Differentiation and identification of Staphylococcus species.
Exercise 3. Differentiation and identification of Streptococcus and Enterococcus
Exercise 4. Written test covering exercises 1-3.
Exercise 5. Non-spore-forming Gram-positive rods, if always threatening the health and life? Listeria and Lactobacillus
Exercise 6. Differentiation and identification of aerobic bacilli - Bacillus
Exercise 7. The clinical significance of aerobic gram-negative rods Pseudomonas and related bacteria.
Exercise 8. Written test covering exercises 5-7
Exercise 9 Isolation and identification of Enterobacteriaceae.
Exercise 10. Anaerobic bacilli – Clostridium
Exercise 11. Yeast infections. Candida and Cryptococcus neoformans
Exercise 12. Written test covering exercises 9-12.
Exercise 13-14. Microbiological safety of medicines, foods, water and air, including the methods of microbiological purity.
Practical test.
Exercise 15. Summary and closing exercises, signature in the index.
Forms of assessment
Note 2: W- Student does not have a knowledge of this subject and has glaring lack of knowledge of English vocabulary; U - does not have the ability to apply the techniques and research tools in the subject; K - Lack of interest in medical microbiology, without active participation during course.
Note 3: W - Student has knowledge of this subject and know the English vocabulary; U - exhibits mediocre ability to apply the techniques and research tools in the subject; K - Is engaged in the learning process; Is aware of treats from pathogenic microorganisms. Basic interest knowledge in pathogenic microorganisms and their diagnosis. Participation during courses.
Note 4: W- Student has knowledge of this subject and has good knowledge of English vocabulary. Knowledge in classification of pathogen microorganisms and their diagnosis; U – shows some ability to apply the techniques and research tools in the subject; K - Achieves the requirements for note 3, is proper engaged for the learning process, attend the courses regularly. Interest in knowledge in pathogenic microorganisms and their diagnosis, active participation in discussions.
Note 5: W - Student has a wide knowledge of this subject and has a very good knowledge of English vocabulary. Strong interest knowledge in pathogen microorganisms and their diagnosis. Participates very actively in discussions; U - demonstrates skills for full application of techniques and research tools in the subject; K - Achieves the requirements for note 4, is highly engaged for the learning process and interested in the new aspect of medical microbiology.

60% - three written tests
25% - Attendance and active participation in course, preparing reports on the course
15% - practical test
Required reading list
Murray P.R., Rosenthal K.S. Pfaller M.A. Medical Microbiology. Elsevier Inc. Philadelphia
Baron S. (ed.) Medical Microbiology. University of Texas Medical Branch at Galveston, Galveston, Texas.
Jorgensen J.H., Pfaller M.A., Carroll K.C., Funke G., Landry M.L., Richter S.S., Warnock D.W. Manual of Clinical Microbiology. Am. Society Microbiology.
Field of study: Biotechnology
Course listing in the Schedule of Courses:
Year/semester:Year II - Semester 4
Number of ECTS credits: 0
Form of assessment: Grade