Molecular biology (lecture) (wykład) - 2019/2020

Course description
General information
Lecturer:prof. dr hab. Ryszard Szyszka
Organising unit:Faculty of Science and Health - Instytut Nauk Biologicznych
Number of hours (week/semester): 2/30
Language of instruction:English
Course objective
Knowledge about the most important processes in organisms (from viruses till mammals) on the molecular level
Prerequisites
passed courses: biochemistry with enzymology, general microbiology
Learning outcomes
KNOWLEDGE
1. The student knows basic terminology used in molecular biology, understands and is able to define basic phenomena and processes undergoing in living organisms (K_W01).
2. The student has knowledge in terms of basic laboratory techniques and research tools used in molecular biology (K_W06).
3. The student has knowledge in terms of genetics and molecular techniques and their practical use, especially in biotechnology (K_W07)

SKILLS
1. The student can independently carry out basic experiments in molecular biology (K_U01, K_U05, K_U07).
2. Student is able to describe a series of experiments carried out in a typical scientific way and interpret the results of experiments (K_U01, K_U05, K_U07).
3. The student knows how to plan and execute a simple experiment (K_U01, K_U05)

SOCIAL COMPETENCE
1. Openminded to modern technologies used in biotechnology (K_K01).
2. The student understands the need to continuous deepening and updating his knowledge and skills (K_K01).
Teaching method
Lecture (powerpoint presentation)
Course content description
DNA as genetic material: definition, structure of prokaryotic and eukaryotic genes, organization of genetic material in pro- and eukaryotic cells, changes in genome (transposition, genetic conversion, amplification of genome fragments. DNA replication: mechanisms of genetic material amplification. Mutagenesis and DNA repair, mechanisms of DNA recombination. Transcription: structure and function of pro- and eukaryotic RNA polymerase, initiation, elongation and termination. Control of gene expression. Potranslational modification of RNA, its regulation and role. Translation: genetic code, initiation, elongation and termination, regulation. Transport of proteins in the cell: mechanisms of transport to specific localisation in the cell. Structure and function of heat-shock protein (HSP). Proteolisis. Signal transduction outside the cell in eu- and prokaryotes. Structure and function of membrane and inner cell receptors. Proteins as molecular transmitters of reaction cascades: G-proteins and Ras, MAP kinase, p53, caspases. Molecular mechanisms of the cell cycle.
Forms of assessment
Grade 2
W - The student does not have a basic knowledge in molecular biology, the place of the discipline among other empirical disciplines. He is not familiar with the current literature and techniques used in molecular biology.
U - The student is not able to analyze and understand the core content of classes. Cannot create his own work tools or use them.
K – The student is not involved in its own learning process, does not fulfill the goals and objectives, is not involved in discussions.

Grade 3
W - The student has a general knowledge about molecular biology, the place of the discipline among other empirical disciplines. Has limited knowledge of the latest research results in the field of molecular biology and techniques used in the field.
U – The student minimally understands content of the classes. With the help of the supervisor analyzes the scientific text and formulates solutions to the problems.
K – The student is attending the classes, but his attitude is passive, devoid of creativity and commitment. To a small extent is involved in discussions and the use of available literature.

Grade 4
W – The student has ordered knowledge in molecular biology, the place of the discipline among other empirical disciplines. He has insights into the latest results of the research and current literature on the subject, knows the basic techniques used in molecular biology.
U - The student is able to present gained knowledge, and can correctly use it in the classroom. With the supervisor help solves the problems.
K - The student actively participates in the laboratory activities, demonstrates openness to the need to deepen knowledge and skills, is involved in discussions.

Grade 5
W - The student has a systematic and established knowledge in molecular biology, the place of the discipline among other empirical disciplines and its historical development. Familiar with the latest research and current literature. He can takes advantage with the gained knowledge in the classroom. Is able to solve problems alone.
U - The student has mastered the tools of analysis and synthesis of knowledge (with reference to the current literature on the subject) and can use them in problematic situations.
K - The student actively participates in the classes, presents initiative and deepens the knowledge and skills using the available literature.

written exam
Required reading list
Basic literature:
1. Alberts B., Johnson A., Levis J., Raff M., Roberts K., Walter P., Molecular Biology of the Cell, New York: Garland Science 2008
2. Brown, T.A. Genomes, 2009
3. Berg, J.M., Tymoczko, J.L., Stryer, L.: Biochemistry, 2009

Other literature:
1. Lodish H., Berk A., Kaiser C.A., Krieger M., Scott M.P., Bretscher A., Ploegh H., Matsudaira P., Molecular Cell Biology, W.H. Freeman Publishers, 2012
Field of study: Biotechnology
Course listing in the Schedule of Courses:
Year/semester:Year II - Semester 4
Number of ECTS credits: 7
Form of assessment: Examination